KMID : 0379520000160020173
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Çѱ¹µ¶¼ºÇÐȸÁö 2000 Volume.16 No. 2 p.173 ~ p.178
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Cytotoxicity on Fibroblast Cells of Several Herbicides
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Rim Yo-Sup
Park Young-Min Jung Yeun-Kyu Han Du-Seok Han Seong-Soo
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Abstract
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This study was carried out to investigate cytotoxicity of several herbicides (Bentazone, Butachlor. Paraquat and Ethalfluralin) in cultured mouse NIH 3T3 fibroblasts. Tetrazolium (MTT), neutral red (NR) and sulforhodamine protein B (SRB) of the colorimetric assays were performed to evaluate the cytotoxicity on cell organelles. 2 x 10^4cell/mell of NIH 3T3 fibroblast in each well of 24 multidish were cultured. After 24 hours, the cells were treated with solution (1, 25, 50 or 100 muM) of each herbicide. After the NIH 3T3 fibroblasts of all groups were cultured in the same condition for 48 hours, MTT, NR and SRB assays were performed to evaluate the cytotoxicity. The light microscopic study was carried out to examine morphological changes of cultured NIH 3T3 fibroblasts. The MTT_{50} of Bentazone, Butachlor, Paraquat and Ethalfluralin were 1560.97 muM, 56.15 mu, 3138.81 muM and 1301.82 muM, respectively. The NR_{50} of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1763.93 muM, 45.98 muM, 1030.85 muM and 1808.29 muM, respectively. The SRB_{50} of Bentazone, Butachlor. Paraquat and Ethalfluralin were 1913.38 muM, 65.30 muM, 1860.73 muM and 1086.93 muM, respectively. The morphological changes of NIH 3T3 fibroblasts showed severe degeneration in Butachlor 50 muM and 100 muM concentrations. These results indicate that Butachlor has high cytotoxicity, Bentazone, Paraquat and Ethalfluralin very weak cytotoxicity against NIH 3T3 fibroblasts.lasts.
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KEYWORD
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Bentazone, Butachlor, Paraquat dichloride, ethalfluralin, NIH 3T3 fibroblast
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